Regulatory
PclpB

Part:BBa_K3773006

Designed by: Justin Berg   Group: iGEM21_William_and_Mary   (2021-10-14)


clpB promoter

This promoter is used as part of a circuit which uses sfGFP to report expression of the promoter. It can therefore be used to indicate a cell's upregulation/downregulation of clpB. It can be placed before the RBS that naturally comes before clpB in E. coli, or alternatively before BBa_B0034 and likely other RBS sequences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 235
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None